Clinical reduction of S. mutans in pre-school children using a novel
liquorice root extract lollipop: a pilot study
M.C. Peters*, J.A. Tallman***, T.M. Braun**, J.J. Jacobson***
To determine the clinical effect of a simple herbal caries-prevention protocol aimed at reduction of Streptococcus mutans (SM) in young children in a pre-school setting.
Proof-of-principle pilot study.
To prove the concept this pilot study delivered a clinical intervention using sugar-free lollipops containing liquorice root extract. Regimen: Supervised herbal lollipops, twice daily for 3 weeks. Species-specific monoclonal antibody testing of saliva provided SM counts. Children were grouped in high, medium and low caries-risk using baseline SM-levels as risk-indicator. Bacterial numbers at baseline, during intervention, and for 9 weeks post-intervention were compared.
SM levels were analyzed using GEE modeling.
High-risk children showed the steepest early decrease in mean log-SM (P<. 001). At end of a follow-up period, the log-SM decrease moved the high-risk group down to moderate-risk level. High-risk children showed a decrease in fitted mean SM% not seen in other groups (P<. 001). The decrease reached a nadir around 22-days post-intervention. Twice-daily use of herbal lollipop significantly reduced both number and relative percent of SM in high-risk children. SM numbers were reduced for 22 days after the last lollipop, stabilized and then began to rebound.
A potential for simple effective caries-prevention for high-risk children has been demonstrated. Encouraging results warrant randomized clinical trials (RCT) of liquorice root in herbal lollipops or alternative modes of delivery.
Eur Archs Paediatr Dent 2010;11(6):274-278
*School of Dentistry,
**School of Public Health, University of Michigan, Ann Arbor,
***Dental Research and Data Institute, Delta Dental of MI, OH, IN, Okemos, Michigan, USA